MAP prevented ultraviolet B (UVB) irradiation-induced immune suppression as determined by contact hypersensitivity (CHS) response in C3H/HeN mice. Under the same conditions, native Aloe barbadensis gel had no effect on macrophage activation. Crude modified Aloe polysaccharide (MAP) activated macrophage cells and stimulated fibroblast growth. The concentration of AVMP fluctuated throughout the year between 940 ± 0 ± 700 ppm, and yielded an annual average of 5500 ± 700 ppm, whereas the aloin fluctuated during the same period between 130 ± 15 and 776 ± 17 ppm, yielding an averageĪloe barbadensis polysaccharide was partially digested with cellulase and further purified by dialysis, stepwise ethanol precipitation, and size exclusion chromatography. Fresh Aloe vera leaves were collected monthly at the home property of Antonio del Rosario (Arinaga, Gran Canaria, Canary For this, an OHpak SB-806 HQ Shodex column was installed in a VWRHitachi, Elite La Chrom liquid chromatograph, equipped with a pump, an automatic injector, a refraction index detector and an UV detector. An analytical method capable of identifying and quantifying has been developed, based on the HPLC technique. The aim of this work was to chemically analyze the fresh juice contained in the plant, so as to identify and quantify the mucopolysaccharide (AVMP) and the aloin (the anthrachinon Aloeemodin, barbaloin and isobarbaloin) on a regular and temporary basis (monthly, for a year). While the first three are beneficial for human health - they stimulate the immune system - the last two produce diarrhea symptoms and, possibly, nephritic problems. The plant produces a colorless extract composed of three fractions of different molecular sized mucopolysaccharides, and two aloin fractions (Aloe-emodin and barbaloin/ isobarbaloin). The Aloe vera plant (Aloe barbadensis Mill.) has been used in functional nutrition for decades.
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